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Perls’ Prussian blue iron staining

CT Carl Maximilian Thielmann
MS Milene Costa da Silva
TM Thomas Muley
MM Michael Meister
EH Esther Herpel
MM Martina U. Muckenthaler
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We used Perls’ Prussian blue staining to determine the tissue iron content of each tumor sample. The staining was performed using the Iron Stain Kit (HT20-1KT, Sigma-Aldrich, St. Louis, MO, USA). Paraffin-embedded tissue samples were rehydrated, stained with Perls’ staining solution for 15 min and rinsed in running tap water for 5 min. The sections were then counterstained with nuclear fast red for 3 min and finally rinsed in running tap water for 5 min. Then, the sections were dehydrated in 70%, 96%, 100% ethanol and xylene and embedded using xylene-based mounting media.

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