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Chromatin Immunoprecipitation (ChIP)
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ChIP was performed, exactly as described previously11. Fold enrichment was calculated with the method of cycle threshold in the formula of 2−ΔΔCT. The primers (forward/reverse) used for the amplicon to CDK1 promoter were 5′- AAAGAAGAACGGAGCGAACA-3′/5′- GCTAGAGCGCGAAAGAAAGA-3′. Control primers (forward/reverse) for a non-binding site (a human DNA alpha satellite) were 5′-TCTCAGAATCTTCCTTTTGATGTG-3′/5′-CCAGTTGCAGATCCTACAAAGA-3′.
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