Statistical analysis

DF Dea Filippini
SA Sabrina D’ Agosto
PD Pietro Delfino
MS Michele Simbolo
GP Geny Piro
BR Borislav Rusev
LV Lisa Veghini
CC Cinzia Cantù
FL Francesca Lupo
SU Stefano Ugel
FS Francesco De Sanctis
VB Vincenzo Bronte
MM Michele Milella
GT Giampaolo Tortora
AS Aldo Scarpa
CC Carmine Carbone
VC Vincenzo Corbo
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Differences between experimental conditions were tested using Student’s t-test or Wilcoxon rank-sum test, according to the normality of samples’ distribution verified with a Shapiro-Wilk test. A p-value < 0.05 was considered statistically significant. Spearman’s rho correlation coefficient method was used to assess correlation between two variables. Survival analyses were performed using the computing environment R50 and the packages survival51, for fitting the model, and survminer52 for plotting. Overall survival data were obtained from the same link reported above. Median survival was estimated with the Kaplan-Meier method and the difference was tested using the log-rank (Mantel-Cox) test. For stratifying survival, gene expression data were divided into quartiles.

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