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Alkaline phosphatase (ALP) activity assay
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After 7 days of culture, ALP activity as an early marker of osteogenic differentiation was assayed utilizing the conversion of a colorless p-nitrophenyl phosphate to colored p-nitrophenol. Cells were washed twice with DPBS and lysed with 0.2% Triton X-100. Cell lysates were assayed for ALP activity using p-nitrophenylphosphate as a substrate. The activity was defined as the amount of p-nitrophenol released after incubation for 30 min at room temperature. Color change was measured spectrometrically at wavelength of 405 nm.
Copyright and License information: The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media B.V., part of Springer Nature ©2018
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