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ApoA1Δ49 was cloned into expression vector PD451-SR. The genes for EGFR were codon optimized for E. coli expression by DNA 2.0, and the SNAP gene was fused to the C-terminal of EGFR. A schematic of EGFR-SNAP, the E. coli codon optimization of human EGFR, and the cDNA and protein sequences of ApoA1Δ49 are shown in Figure S1. The fused EGFR-SNAP was cloned into expression vector pJexpress414 (DNA 2.0)
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