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The transgenic poplar was obtained by leaf disk method (Nehra et al., 1990; Nietsch et al., 2017). Poplar leaves from 1-month-old seedlings were cut to 1 cm x 1 cm leaf disks and put on MS differentiation medium containing 50 mg/L kanamycin and 200 mg/L cephalosporin. The seedlings were then grown on rooting medium containing 50 mg/L kanamycin. The specific primers were designed to detect the positive transgenic poplars by both polymerase chain reaction (PCR) and reverse transcriptase PCR (RT-PCR).
Copyright and License information: ©2019 Cheng, Zhang, Zhao, Yao, Li, Zhou and Jiang
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