2.2. Pheromone Extraction

Sex pheromone glands were removed from 3- to 5-day-old virgin female moths after approximately 6–7 h into the scotophase. The gland was everted by gentle pressure on the abdomen, gripped with the forceps and sliced from the abdomen with a razor blade such that the gland remained inflated. The glands were then dipped into a glass vial containing approximately 200 μL of redistilled hexane for 30 s. These extracts (8–10 glands per extract) were transferred into glass capillaries with pointed bases, concentrated under a slow stream of high purity nitrogen to about 20 μL, and stored at −20 °C for future analysis.