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DPPH radical scavenging assay

ZN Zofia Nizioł-Łukaszewska
DF Dominika Furman-Toczek
MZ Martyna Zagórska-Dziok
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Antioxidant activity of plant extract was analysed using DPPH free radical scavenging assay, according to the method described by Brand-Williams et al. [25]. 167 μL of 4 mM ethanol solution of DPPH was mixed with 33 μL analysed samples in different concentrations (250 μg·ml− 1 – 5000 μg·ml− 1). The absorbance was measured at λ = 516 nm in every 5 min for 30 min using UV-Vis spectrophotometer Filter Max 5 (Thermo Scientific). DPPH solution mixed with equal volume of distilled water was served as a control. The percentage of the DPPH radical scavenging were calculated using the equation:

Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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