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Detergent Fiber Analysis (DFA)

LR Luis A. Rivera-Burgos
JV Jeffrey J. Volenec
GE Gebisa Ejeta
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Hemicellulose and cellulose of stover of 236 RIL were analyzed by modified filter bag technology using ANKOM 2000 instrument (ANKOM Tech., Corp., NY) (Vogel et al., 1999; Wu et al., 2006; Lemus et al., 2008; Anderson et al., 2009). Two modifications were made for neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) analyses: a) two empty bags were included in each run as an indicator of bag mass loss during the extraction; one empty bag was placed in the first tray, and the other in last tray to determine the blank bag correction; b) filter bags were dried overnight at 45ºC before to proceeding to next analysis step. Hemicellulose concentration was calculated by subtracting ADF concentration from the NDF concentration. Cellulose concentration was calculated by subtracting ADL concentration from the ADF concentration. Finally, lignin concentration was determined as mass loss following the ashing of the ADF.

Copyright and License information:  ©2019 Rivera-Burgos, Volenec and Ejeta
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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