Apoptosis assay and cell cycle assay

YZ Yancheng Zhong
SY Sheng Yang
WW Wei Wang
PW Pingpin Wei
SH Shiwei He
HM Haotian Ma
JY Juan Yang
QW Qian Wang
LC Lanqin Cao
WX Wei Xiong
MZ Ming Zhou
GL Guiyuan Li
CS Cijun Shuai
SP Shuping Peng
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Stable OC cell lines or transfected OC cells were used for analysis. Apoptosis assay was examined using the annexin VFITC/PI Apoptosis Detection Kit (BIOBOX, China) following to the reagent’s instructions. And for cell cycle detection, OC cells were re-suspended by 300 μl PBS, then 700 μl cooled ethanol was added to the cell suspension, and was fixed for at least 12 h at −20 °C. Follow the steps in the reagent’s instructions for the Cell Cycle Staining Kit (Multi Science, China).

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