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DNI measurements

TK Taeyoung Kong
YP Yoo Seok Park
HL Hye Sun Lee
SK Sinae Kim
JL Jong Wook Lee
JY Je Sung You
HC Hyun Soo Chung
IP Incheol Park
SC Sung Phil Chung
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The specific analyzer performs DNI measurements using two different systems based on a cytochemical myeloperoxidase tungsten-halogen channel and a laser diode channel8–10. The cytochemical myeloperoxidase tungsten-halogen channel measures and differentiates neutrophils, eosinophils, lymphocytes, monocytes, and large unstained cells based on size and myeloperoxidase staining intensity8–10. The laser diode channel calculates, classifies, and counts cell types according to lobularity/nuclear density and size8–10,12. The DNI is then calculated by subtracting the fraction of mature polymorphonuclear neutrophils from the sum of the myeloperoxidase-reactive cells, detecting circulating immature granulocytes as the leukocyte subfraction11–13.

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