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To investigate the stimulation effect of COS on various biological function gene sets in macrophages, differences in gene mRNA expression levels of biological functional annotation and pathways between control and COS stimulation groups with different MW were analyzed by GSEA (http://software.broadinstitute.org/gsea/downloads.jsp). For use with GSEA software, the database file (Dataset S5) about KEGG pathway enrichment analyses of M. amblycephala was built. P-value < 0.05 was chosen as the cut-off criteria.

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