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Measurements of extracellular acidification rate (ECAR) and oxygen consumption rate (OCR)

PP Paola Pellegrini
JS Jason T. Serviss
TL Thomas Lundbäck
NB Nicolo Bancaro
MM Magdalena Mazurkiewicz
IK Iryna Kolosenko
DY Di Yu
MH Martin Haraldsson
PD Padraig D’Arcy
SL Stig Linder
AM Angelo De Milito
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HCT116 cells were plated at 60,000 cells/well in 100 µl medium in XF24-well cell plates. Subsequently medium was replaced with 500 µl Seahorse assay medium (1 mM pyruvate, 25 mM glucose and 2 mM glutamine). Oligomycin, FCCP, rotenone and antimycin A were injected according to the Seahorse XF cell mito stress kit. The mitochondrial function was assessed detecting the ECAR and OCR values by using the Seahorse XF analyzer.

Copyright and License information: The Author(s) ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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