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Cecal Ligation and Puncture (CLP) Sepsis Animal Model
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Sepsis was induced by cecal ligation and puncture (CLP) as described previously (32). Briefly, the mice were anesthetized with pelltobarbitalum natricum and shaved an incision in the abdomen. The cecum was exposed and ligated distal to the cecum. The cecum was punctured with an 18-g needle, and a small amount of cecum content was extruded. Then the cecum was replaced into the abdominal cavity, and the incision was closed.
Copyright and License information: ©2019 Wang, Wang, Wu, Liu, Zhou, Mi, Zhang and Wang.
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