2.8 Loading of β-Peptide into Film-Coated Catheters

A 10 mg/mL solution of β-peptide 1 in 0.15 M NaCl was infused into tubes coated with HA/CH or PGA/PLL multilayers. The peptide solution was allowed to remain in the tubes at room temperature for 24 hours. The peptide solution was then removed from the tubes and the tubes were rinsed consecutively using a solution of PBS for 5 min, a solution of Tris-buffered saline Tween (TBST; 10 mM Tris–HCl, 100 mM NaCl, 0.1% Tween–20) for 1 hour, and a solution of PBS for 5 min. Tubes fabricated using this procedure were either used immediately or stored in a dried state at room temperature until use. Control uncoated tubes (no multilayer coating) were also treated with peptide in the manner described above. Film-coated control tubes (no peptide) were fabricated as described above but were not loaded with β-peptide.