4.3. Solid-Phase Peptide Synthesis

Peptides were manually synthesized by the solid-phase approach using Fmoc/t-butyl chemistry.³⁷ Peptide elongation was carried out in polypropylene syringes fitted with a polyethylene porous disk. Solvents and soluble reagents were removed by suction. A Wang Resin and Rink amide MBHA Resin (Peptides International) were used for C-terminal carboxylated and amidated peptide synthesis respectively. Samples were treated with trifluoroacetic acid (TFA)/triisopropylsilane/water (95:2.5:2.5) for removal of the protecting group and cleavage. Identity of the peptide was confirmed by electrospray MS on a MicroTOF Q II (Bruker Daltonics).