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Western Blot Analysis of Protein Expression
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Expression of anti-apoptotic factors and other regulatory molecules was evaluated according to the standard protocols. Protein concentration was determined using a Bradford/Bio-Rad Protein Assay (Bio-Rad, Hercules, CA), based on bovine serum albumin standard curve. Blots were detected using SuperSignal West Pico Chemiluminescent Substrate (ThermoScientific, Waltham, MA), and band densities were quantified using LI-Cor Image Studio Lite software (LI-Cor Biotechnology, Lincoln, NE).
Copyright and License information: The Author(s) ©2018
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