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Spindle indices (SI) of individual cells were calculated from at least 200 cells per treatment from a minimum of three images as the ratio of length (l) to width (w); SI = l/w of each cell. Cells with SI < 3 were considered as epithelial. The percentage of cells <3 were calculated as the ratio of the number of cells with spindle index <3 to the total number of cells per image. The method was adopted from reference (30). Length and width were measured using the Image J software, U. S. National Institutes of Health, Bethesda, Maryland, USA.
Copyright and License information: ©2019 Bhatt, Gupta, Hoang, Chakrabarty, Wright, Elliot, Chopra, Monlish, Anna, Burow, Cavanaugh and Flaherty.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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