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Generation of Tamoxifen-Resistant MCF-7 Cell Line
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The MCF-7 cells were cultured in phenol red-free RPMI-1640 media and 5% charcoal-stripped FBS (to remove endogenously expressed protein growth factors present in the media) in the presence of DMSO or (Z)-4-Hydroxytamoxifen (4-OHT) (Sigma-Aldrich Cat. No. H7904) at a concentration of 0.1 μM for 6 months.
Copyright and License information: ©2019 Bhatt, Gupta, Hoang, Chakrabarty, Wright, Elliot, Chopra, Monlish, Anna, Burow, Cavanaugh and Flaherty.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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