Total antioxidant capacity

The phosphomolybdenum method was used to measure the total antioxidant capacity of the extract. 0.1 ml of standard/extract solution was mixed with 0.3 ml of reagent (4 mM ammonium molybdate, 0.6 M sulfuric acid with 28 mM sodium phosphate) and kept for 90 min at 95 °C. The mixture was allowed to attain room temperature, then absorbance was measured at 695 nm. Ascorbic acid was used to obtain the calibration curve, and represented as ascorbic acid equivalents.