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Dual-luciferase reporter assay

JL Jie Li
JD Jie Dong
SL Shaohua Li
WX Wei Xia
XS Xueting Su
XQ Xingliang Qin
YC Ying Chen
HD Hongmei Ding
HL Hui Li
AH Aixue Huang
CB Chenjun Bai
TH Tongnan Hu
CW Chenglong Wang
BC Bingfeng Chu
NS Ningsheng Shao
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Reporter plasmids containing the WT or mutated 3′UTRs and control plasmid [cytomegalovirus (CMV)–driven Renilla luciferase construct, pRL-CMV] (Promega), and miRNA mimics or negative control mimics were co-transfected into cells using Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions. After 48 h, the reporter activity was measured using a dual-luciferase reporter gene assay kit (Promega).

Copyright and License information: The Author(s) ©2017
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