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2.5. Serum cholesterol

DG Daniel A. Giles
BR Bhama Ramkhelawon
ED Elizabeth M. Donelan
TS Traci E. Stankiewicz
SH Susan B. Hutchison
RM Rajib Mukherjee
MC Monica Cappelletti
RK Rebekah Karns
CK Christopher L. Karp
KM Kathryn J. Moore
SD Senad Divanovic
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Serum was initially diluted 1:10 in saline and 25 μL of diluted serum or standard was then added to a 96 well clear flat bottom plate (Costar) containing 100 μL of Infinity Cholesterol Liquid Stable Reagent (Thermo Scientific). Standards (Thermo Scientific) were prepared according to manufacturer's instructions. Serum cholesterol was quantified at 500–520 nm using Molecule Devices vmax and Kinetic Microplate Reader and SoftMax Pro v5 software.

Copyright and License information:  ©2016 The Author(s)
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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