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Immunohistochemical analysis of Ki-67 expression in mouse xenografts
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Mouse xenograft tumors were placed in fresh formalin for a minimum of 24 hours before processing for paraffin embedding. Sections were cut at 5 μm and mounted on glass slides. Slides were deparaffinized and hydrated in Xylene, gradually diluted in ethanol and water followed by antigen retrieval in Sodium Citrate at pH 6. Slides were probed with 1:2000 diluted Ki67 (AbCam) at 4C overnight, then washed and incubated with HRP conjugated secondary antibody. Antibody complexes were detected with 3,3′-diaminobenzidine and counter stained with Mayer's Hematoxylin.
Copyright and License information: ©2016 Divine et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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