Tail bleeding time

The Rgs10^-/- and WT mice were used for the tail bleeding assay. Hemostasis was examined using the tail transection technique [13–16]. Briefly, mice were anesthetized with isoflurane and place on a 37 ^oC homeothermic blanket and their tails were transected 5 mm from the tip. The tail was placed in saline at 37 ^oC and the time to bleeding cessation was measured. Bleeding stoppage was not considered complete until bleeding had stopped for 1 min. When required, measurements were terminated at 15 min.