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Cytotoxicity assay in vitro

YG Yan Ge
HW Haiyan Wang
JR Jinghua Ren
WL Weilin Liu
LC Lingjuan Chen
HC Hongqi Chen
JY Junjie Ye
ED Enyong Dai
CM Congrong Ma
SJ Songguang Ju
ZG Zong Sheng Guo
ZL Zuqiang Liu
DB David L Bartlett
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Tumor cells were plated at 1.0×104 (except B16 cells, which were plated at 5×103) cells per well in 96-well plates and infected with indicated viruses the next day at different MOIs. Cell viability was determined at 48 hours after infection using CellTiter 96 Aqueous Nonradioactive Cell Proliferation Assay (Promega, Madison, Mississippi) or Cell Counting Kit-8 (Boster Biological Technology, Pleasanton, California).

Copyright and License information:  ©2020-03-24 Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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