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Histopathology and immunohistochemistry

YZ Yanyan Zhang
ES Erika Saavedra
RT Ruoping Tang
YG Yin Gu
PL Patrick Lappin
DT Dusko Trajkovic
SL Shu-Hui Liu
TS Tod Smeal
VF Valeria Fantin
SB Stephane De Botton
OL Ollivier Legrand
FD Francois Delhommeau
FP Flavia Pernasetti
FL Fawzia Louache
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At the end of treatment, mice were euthanized and the femur, spleen and liver were collected from each mouse and preserved in 10% neutral buffered formalin. Fixed tissues were processed to paraffin block and sectioned. Subsequent slides were stained with hematoxylin and eosin (H&E) and evaluated for changes in morphology. Additional sections from tissue sample were stained immunohistochemically with anti-human mitochondria membrane antibody (Millipore, MAB1273) at 1 μg/mL. The sections were counterstained with hematoxylin and examined by a Zeiss Axiophot microscope. Photos were acquired at 10X magnification for large panels and 40X magnification for the inserts.

Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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