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RINm5F cells seeded in 96-well microplate were cultured at 37 °C in a humidified atmosphere for 72 h, and then the cells were exposed to different treatments for different periods of time. After incubation, 20 μL/well of MTT solution (5 mg/mL in PBS buffer) was added and incubated for 4 h. The medium was aspirated and replaced with DMSO to dissolve the formazan salt. The color intensity of the formazan solution, which reflects the cell growth condition, was measured at 570 nm using a microplate spectrophotometer.
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