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Luciferase Reporter Assay

XC Xi-Sha Chen
KW Kuan-Song Wang
WG Wei Guo
LL Lan-Ya Li
PY Pian Yu
XS Xin-Yuan Sun
HW Hai-Yan Wang
YG Yi-Di Guan
YT Yong-Guang Tao
BD Bo-Ni Ding
MY Ming-Zhu Yin
XR Xing-Cong Ren
YZ Yi Zhang
CC Ce-Shi Chen
YY Yuan-Chao Ye
JY Jin-Ming Yang
YC Yan Cheng
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To analyze ERE promoter activity, HCC1806 cells stably expressing the indicated shRNA were co-transfected with the ERE-containing luciferase reporter plasmid or identical NON-Luc construct plus pRL-TK-Luc to assess transfection efficiency, and then treated with 10nM E2 for 24h. Cells were harvested and the luciferase activities were measured using Promega's Luciferase Assay System (Promega, Madison, Wisconsin). The luciferase values (relative light units, RLUs) were normalized based on the activity of pRL-TK-Luc.

Copyright and License information:  ©2020-01-01 The author(s)
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