2.6. Inhibition of protein denaturation

The effect of date seeds extracts on protein denaturation was evaluated based on the method described by Hmidani et al. (2019). The reaction mix made of 1 mL date seed extracts at different doses (20–1000 μg/mL) and 1 mL of 1% bovine albumin prepared in phosphate-buffered saline (PBS, pH 6.4). The reaction mixtures were left at 37 °C for 20 min and then heated in a shaking water bath at 70 °C for 5 min. After cooling, the reaction mixtures turbidity was determined at 660 nm. The control and standard were prepared in a similar manner as was done for the test samples using buffer and diclofenac sodium (20–1000 μg/mL) instead of extract. The percent inhibition of protein denaturation (%IPD) was calculated as follows:

Abs control and Abs sample are the absorbance buffer and the absorbance of sample extract/standard, respectively. Then IC₅₀ value that represents the concentration of drug or extracts required to inhibit protein denaturation by 50% was determined.