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2.8. Autophagy Flux Assay Using RFP-GFP-LC3 Construct
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A549 cells incubated on a cell culture slide (SPL. Lot No. BA5A09A, Korea) were transfected with green fluorescent protein-labeled LC3 (GFP-LC3) or red-green fluorescent protein-labeled LC3 (RFP-GFP LC3) using X-treme GENE HP DNA Transfection Reagent (Roche, Lot. 11062400, Switzerland). The fluorescent color was analyzed in Misaponin B-treated A549 cells for 24 h by fluorescence microscopy.
Copyright and License information: ©2020 Gunho Won et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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