Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
The concentration of alkaline phosphatase (ALP) in cells was measured after 3, 14 and 30 days of culture using p-nitrophenyl phosphate as a phosphatase substrate in a commercial Alkaline Phosphatase colorimetric assay Kit (Sigma Chemical, St. Louis, MO, USA) at a wavelength 405 nm according to the manufacturer’s instructions. The ALP concentration was subsequently normalised to the total protein content (Quick Start Bradford protein assay, Bio-rad, Australia) of each sample.
Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
