Chymase mRNA production by in situ hybridization

Brittany Butts; Mustafa I. Ahmed; Navkaranbir S. Bajaj; Pamela Cox Powell; Betty Pat; Silvio Litovsky; Himanshu Gupta; Steven G. Lloyd; Thomas S. Denney; Xiaoxia Zhang; Inmaculada Aban; Sakthivel Sadayappan; James W. McNamara; Michael J. Watson; Carlos M. Ferrario; James F. Collawn; Clifton Lewis; James E. Davies; Louis J. Dell’Italia

Improve Research Reproducibility A Bio-protocol resource

Home
Protocols

Concise Method

Chymase mRNA production by in situ hybridization

BB Brittany Butts

MA Mustafa I. Ahmed

NB Navkaranbir S. Bajaj

PP Pamela Cox Powell

BP Betty Pat

SL Silvio Litovsky

HG Himanshu Gupta

SL Steven G. Lloyd

TD Thomas S. Denney

XZ Xiaoxia Zhang

IA Inmaculada Aban

SS Sakthivel Sadayappan

JM James W. McNamara

MW Michael J. Watson

CF Carlos M. Ferrario

JC James F. Collawn

CL Clifton Lewis

JD James E. Davies

LD Louis J. Dell’Italia

This method is extracted from research article: JACC Basic Transl Sci, Jan 2020

Reduced Left Atrial Emptying Fraction and Chymase Activation in Pathophysiology of Primary Mitral Regurgitation

DOI: 10.1016/j.jacbts.2019.11.006

Ask a question

Favorite

The expression of human chymase (CMA1) mRNA was measured by in situ hybridization as previously described by our laboratory (13). For details, see the Supplemental Appendix.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

Post a Question

0 Q&A

Share your protocol with your peers.

Submit a Preprint Protocol