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Enzyme‐linked immunosorbent assay (ELISA) was used to evaluate tumor necrosis factor‐alpha (TNF‐α) and interleukin‐6 (IL‐6) expression. The RAW 264.7 cells (1 × 106 cells/ml) were aliquot into six‐well plate and incubated for overnight; 1 μg/ml lipopolysaccharide (LPS) was added to 250 μg/ml of each sample. After incubation for 24 hr, the concentration of TNF‐α and IL‐6 in the cell‐free supernatant (i.e., CM) was assessed by using the ELISA kit (Cloud‐Clone Crop) according to the manufacturer's instructions.
Copyright and License information: ©2020 The Authors. published by Wiley Periodicals, Inc.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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