2.6. Pharmacokinetic Studies in Mice

Test compounds were administered to mice by oral gavage followed by blood sampling at intervals of 30, 60, 120, 240, 360, 480, and 1440 min [5,7]. Compounds were dosed orally at 50 mg/kg in 0.2 mL of dosing solution (7% Tween 80, 3% ethanol, 5% DMSO, 0.9% saline). Experiments were performed with groups of three mice per compound as published. Plasma was separated and extracted with acetonitrile for measurements of the compound concentrations by liquid chromatography/tandem mass spectrometry.