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Transwell chambers (24-well; Costar, Boston, MA, U.S.A.) were applied for cell migration assay. Cells in serum-free media were put into upper chambers afterwards, while medium with 10% FBS was put into the bottom chambers. After incubation for 24 h, migratory cells were treated with 4% PFA (Sigma-Aldrich, Burlington, Massachusett, U.S.A.) and 0.5% Crystal Violet. The cell numbers of three replications were counted in five different fields by a microscope.
Copyright and License information: ©2020 The Author(s).
This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
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