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HTS results of RNA sequencing were aligned to the reference plasmid pACYC184_CcCas9_locus using BWA aligner (13). Determined coordinates of 5′ and 3′ RNA ends were used to reconstruct the full-length RNA sequences. The resulting fragments were analyzed using Geneious 11.1.2. Filtered 40–130 nt-length sequences were used to generate the alignment.
Copyright and License information: ©2020 The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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