Drosophila embryo injections

All plasmids used for embryo injections were extracted from transformed Escherichia coli cells using a plasmid Midiprep kit from Thermo Fisher Scientific. To disrupt the Nopp140 gene, the plasmid injection mixture contained 15 ng µl⁻¹ of gRNA#52, 15 ng µl⁻¹ of gRNA#99, and 230 ng µl⁻¹ of pDsRed-Donor. The mixture was injected into homozygous nanos-Cas9 transgenic embryos. To disrupt the DsRed gene, the CRISPR injection mixture contained 75 ng µl⁻¹ of gRNA#2, 75 ng µl⁻¹ of gRNA#3, and 350 ng µl⁻¹ of pBS-Hsp70-Cas9. This mixture was injected into J11 DsRed/TM3-GFP embryos. All injections were performed by GenetiVision Corporation (Houston, TX, USA).