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Human melanoma A375p (CRL-1619) and colon cancer HCT 116 (CCL-247) cell lines were obtained from American Type Culture Collection while the human melanoma cell line A375-M6 was isolated from lung metastasis of SCID bg/bg mice IV injected with A375p [21] and grown in DMEM with 10% fetal bovine serum (Euroclone, Milano, Italy). Cells were tested every two weeks for Mycoplasma by PCR using two universal primers (MGSO and GPO1) [22].
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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