4.8. Deconvolution of Amide I Region (1600–1700 cm−1)

JD Joanna Depciuch
BK Bartosz Klębowski
MS Małgorzata Stec
RS Rafał Szatanek
KW Kazimierz Węglarczyk
MB Monika Baj-Krzyworzeka
MP Magdalena Parlińska-Wojtan
JB Jarek Baran
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The secondary protein structure was analyzed by means of curve fitting using MagicPlot software (Magicplot Systems, Saint Petersburg, Russia). First, the second derivative spectra were calculated based on the ATR-FTIR spectra to determine the initial peak positioning for the curve fitting. The peaks were fitted using a Gaussian function and the area under the curve was considered at 100%. Each component was expressed as its percentage after fitting.

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