4.3. Evaluation of the Impacts of AECV and EAECV on RBCs of Cattle and Horse

The hemolytic efficacy of AECV and EAECV on cattle and horse RBCs was evaluated in vitro as previously reported elsewhere [6,42]. Initially, AECV and EAECV extracts at 600 µg/mL were cultivated at 37 °C with purified bovine and equine RBCs for 3 h. Afterward, the pretreated-RBCs were washed three times with PBS and mixed with bovine and equine parasites. To monitor the parasitemia and any side effects as a result of the pretreatment, Giemsa-stained blood smears were prepared daily.