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The PC3, DU145, and LNCaP (prostate carcinoma) cell lines were purchased from American Type Culture (ATCC®). Cells were kept for 72 h in 175 cm2 flasks at 310 K in a humidified atmosphere with 5% CO2 in the recommended culture media as suggested by ATCC, in particular: LNCaP: RPMI1640 (D-glucose 25.0 mM); PC3: F-12K (D-glucose 7.0 mM); DU145: EMEM (D-glucose 5.6 mM). All the cells were used within the first 10 passages from unfreezing.
Copyright and License information: ©2020 Cavallari, Carrera, Di Matteo, Bondar, Aime and Reineri.
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