2.6. Immunohistochemistry

Animals were euthanized at the end of the experiment and pancreata harvested, embedded in OCT compound (Tissue Tek, Torrance, CA, USA) or paraffin, and stained for insulin using a rat anti-insulin primary antibody ((1:100, #MAB1417, R & D systems, Minneapolis, MN, USA) and a donkey anti-rat IgG secondary antibody conjugated to Alexa488 (1:500, Invitrogen, Carlsbad, CA, USA) as previously described [10].