2.6. Determination of cyclobutane pyrimidine dimer (CPD) photoproducts by ELISA

Saowanee Jeayeng; Adisak Wongkajornsilp; Andrzej T. Slominski; Siwanon Jirawatnotai; Somponnat Sampattavanich; Uraiwan Panich

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2.6. Determination of cyclobutane pyrimidine dimer (CPD) photoproducts by ELISA

SJ Saowanee Jeayeng

AW Adisak Wongkajornsilp

AS Andrzej T. Slominski

SJ Siwanon Jirawatnotai

SS Somponnat Sampattavanich

UP Uraiwan Panich

This method is extracted from research article: Free Radic Biol Med, May 2017

Nrf2 in keratinocytes modulates UVB-induced DNA damage and apoptosis in melanocytes through MAPK signaling

DOI: 10.1016/j.freeradbiomed.2017.05.009

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MC were irradiated with UVB (125 mJ/cm²) and harvested at 1 h post-irradiation. Genomic DNA was then extracted using a spin-column based genomic DNA isolation kit (GET^™ DNA Template) (G-Bioscience, St Louis, MO, USA) according to the protocol’s instruction. The CPD in genomic DNA was measured by Oxiselect oxidative DNA damage ELISA kit (Cell Biolabs, San Diego, CA). The CPD was detected with an anti-CPD antibody, followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibody.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/)

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