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Preparation of the modified G-250 dye and ninhydrin reagent
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The G-250 dye was prepared according to the modified method described by [25]. Two hundred milligram of G-250 was dissolved in 40 mL ethanol. Then, 320 mL of 50% ethanol in distilled water was prepared and 40 mL of orthophosphoric acid was added. The ninhydrin reagent was 0.2 g ninhydrin in 100 mL of ethanol.
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