Beta amyloid (Aβ) enzyme linked immunoabsorbant assays

Two individual rodent-specific Aβ_1-42 enzyme linked immunoabsorbant assays (ELISAs) were performed. One assay utilized a ThermoFisher mouse endogenous Aβ_1-42 ELISA kit, and the other a Wako human/rat/mouse high sensitivity endogenous Aβ_1-42 ELISA kit. Briefly, cortical tissue was homogenized in 8 M guanidine hydrochloride with protease inhibitor cocktail (ThermoFisher), and incubated at room temperature while mixing by inversion for 4 hours. For both ELISA kits samples were diluted 1:5. Values were normalized to protein content.