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Short-chain fatty acid derivatization.

OJ Omari Jones-Nelson
AT Andrey Tovchigrechko
MG Matthew S. Glover
FF Fiona Fernandes
UR Udaya Rangaswamy
HL Hui Liu
DT David E. Tabor
JB Jonathan Boyd
PW Paul Warrener
JM Jose Martinez
JH Jamese J. Hilliard
CS C. Ken Stover
WY Wen Yu
GD Gina DAngelo
SH Sonja Hess
TC Taylor S. Cohen
BS Bret R. Sellman
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SCFAs were derivatized with 3-nitrophenylhydrazine (3-NPH) based on a method previously described by Borchers and coworkers (63). Fecal extracts (20 μl) were sequentially mixed with 10 μl of 200 mM 3-NPH–HCl in 50:50 acetonitrile-water (ACN-H2O) solution and 10 μl of 120 mM N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide–HCl (EDC) in 50:44:6 ACN-H2O-pyridine solution. Mixtures were reacted at 40°C with 800 rpm mixing for 30 min. 3-NPH-derivatized samples were diluted with 960 μl of 50:50 ACN-H2O. One hundred microliters of 3-NPH-derivatized samples was mixed with 100 μl 13C6-3-NPH-derivatized SCFA internal standard containing derivatized acetic acid, propionic acid, isobutyric acid, butyric acid, 2-methyl butyric acid, isovaleric acid, and valeric acid.

Copyright and License information:  ©2020 Jones-Nelson et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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