2.1. Fungal Isolates

The Basidiomycota isolates of the study are stored in the Fungal Biotechnology Culture Collection of the Microbial Domain Biological Resource Centre HAMBI (HAMBI mBRC, Helsinki, Finland), which is a part of the Biodiversity Collections Research Infrastructure in the Helsinki Institute of Life Science, University of Helsinki. The fungi belong to the taxonomic class Agaricomycetes order Polyporales (Phlebia radiata and Fomitopsis pinicola) and order Agaricales (Schizophyllum commune and Coprinopsis cinerea) and have their distinct decay strategies and lignocellulose substrate specificities (Table 1). S. commune H4-8 homokaryon [20] was obtained from H. Wösten, and C. cinerea Amut Bmut homokaryon [21] from L. Nagy, whereas the P. radiata and F. pinicola wild-type isolates originate from Finland [12,22]. Fungi were first cultivated on 2% (w/v) malt extract (Biokar Diagnostics, France) agar (MEA) plates at 25 °C in the dark for 7 days. The species have been genome-sequenced with annotated genomes available at the DOE Joint Genome Institute (JGI) MycoCosm genome repository [23].

Fungal isolates used in this study.

¹ WR, white rot; BR, brown rot; “GR”, intermediate grey rot; LDF, litter-decomposing fungus. ² D, deciduous; C, coniferous; LC, lignocellulose.