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Yeast cells were grown under the conditions described above, harvested at early exponential phase, suspended in PBS, incubated with 0.02% (w/v) Calcofluor White (CFW, Fluka, St. Louis, MO) for 30 min at room temperature, and washed 3 times with PBS. Cells were observed using a Leica Microsystems DM-5000B epifluorescence microscope with appropriate filter settings using a 100x oil-immersion objective. Images were acquired with a Leica DCF350FX digital camera and processed with LAS AF Leica Microsystems software.
Copyright and License information: ©2020 António Rego et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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