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Transfected MCF7 and MDA-MB-175-VII cells were seeded in triplicate in 96-well plates at density of 2000 cells per well. The following day, cells were treated with increasing concentrations of 4-Hydroxytamoxifen (2–15 μM). At 72 h post-incubation, cell viability was measured by MTS assay using Infinite F50 (Tecan, Männedorf, Switzerland).
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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