Mouse infection studies

JH Julia A. Horstmann
ML Michele Lunelli
HC Hélène Cazzola
JH Johannes Heidemann
CK Caroline Kühne
PS Pascal Steffen
SS Sandra Szefs
CR Claire Rossi
RL Ravi K. Lokareddy
CW Chu Wang
LL Laurine Lemaire
KH Kelly T. Hughes
CU Charlotte Uetrecht
HS Hartmut Schlüter
GG Guntram A. Grassl
TS Theresia E. B. Stradal
YR Yannick Rossez
MK Michael Kolbe
ME Marc Erhardt
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Seven-week-old C57BL/6 mice (Janvier) were pretreated with 100 mg mL−1 streptomycin. Mice were co-infected intragastrically with 107 colony forming units (CFU) each of two Salmonella Typhimurium strains that contained a different antibiotic resistance cassette. Small intestine, cecum and colon were isolated 2 days post-infection and plated on respective antibiotics resistance plates. The CFU were counted and reported as CFU per gram tissue. Competitive indices (CI) were calculated by normalizing the percentage of each strain to the inoculum and the challenge strain.

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